Protein-DNA interactions of oxidative-stress transcription factors OxyR1 and OxyR2 in Aliivibrio fischeri
AdvisorRodriguez-Martínez, José A.
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Transcription factors (TFs) are regulatory proteins that bind tightly to specific DNA sequences 15-20 base pairs long. Being sequence-specific DNA-binding proteins, TFs bear the key to the cellular state and control how organisms respond to different environmental stresses. OxyR, a LysR-type transcription factor, binds strongly to H2O2 and activates a set of genes whose main purpose is to protect bacteria against oxidative stress. The genome of Aliivibrio fischeri bacteria codes for two different OxyR proteins, OxyR1 and OxyR2. However, the collection of target genes of OxyR1 and OxyR2 in A. fischeri remains to be determined. This study aims to clone, overexpress, and purify OxyR1 and OxyR2 TFs to determine their DNA binding functionality throughout the assessment of their intrinsic DNA-binding preferences. Purified OxyR1 and OxyR2 TFs were used to determine their DNA binding specificity using Systematic Evolution of Ligands by Exponential Enrichment (SELEX-seq).