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dc.contributor.advisorRodriguez-Martínez, José A.
dc.contributor.authorCastro-Martínez, Alexander
dc.description.abstractTranscription factors (TFs) are regulatory proteins that bind tightly to specific DNA sequences 15-20 base pairs long. Being sequence-specific DNA-binding proteins, TFs bear the key to the cellular state and control how organisms respond to different environmental stresses. OxyR, a LysR-type transcription factor, binds strongly to H<sub>2</sub>O<sub>2</sub> and activates a set of genes whose main purpose is to protect bacteria against oxidative stress. The genome of <em>Aliivibrio fischeri</em> bacteria codes for two different OxyR proteins, OxyR1 and OxyR2. However, the collection of target genes of OxyR1 and OxyR2 in <em>A. fischeri </em>remains to be determined. This study aims to clone, overexpress, and purify OxyR1 and OxyR2 TFs to determine their DNA binding functionality throughout the assessment of their intrinsic DNA-binding preferences. Purified OxyR1 and OxyR2 TFs were used to determine their DNA binding specificity using Systematic Evolution of Ligands by Exponential Enrichment (SELEX-seq).en_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.subjectAliivibrio fischerien_US
dc.subjectDNA binding specificityen_US
dc.subjectSystematic Evolution of Ligands by Exponential Enrichment (SELEX-seq)en_US
dc.subject.lcshBacterial geneticsen_US
dc.subject.lcshDNA-binding proteinsen_US
dc.subject.lcshDNA-protein interactionsen_US
dc.subject.lcshOxidative stressen_US
dc.subject.lcshTranscription factorsen_US
dc.titleProtein-DNA interactions of oxidative-stress transcription factors OxyR1 and OxyR2 in <em>Aliivibrio fischeri</em>en_US
dc.rights.holder© 2022 Alexander Castro Martínezen_US
dc.contributor.committeePeterson-Peguero, Esther
dc.contributor.committeeBaerga-Ortiz, Abel
dc.contributor.committeeToranzos, Gary A.
dc.contributor.campusUniversity of Puerto Rico, Río Piedras Campusen_US
dc.description.graduationSemesterSpring (2nd Semester)en_US

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Attribution-NonCommercial-NoDerivs 3.0 United States
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