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Now showing items 921-930 of 1298
Tiempo y angustia de los personajes frente al binomio vida-muerte en la narrativa de Edgardo Sanabria Santaliz
(2023-05-05)
El trinomio tiempo-finitud-angustia son conceptos vinculados a la muerte, al dolor, al temor y al sufrimiento. La muerte representa una experiencia de crisis para la persona que, ya sea por la conciencia de finitud o por ...
Prácticas políticas, nociones de género y la articulación del poder en el espacio de la primera dama del Estado Libre Asociado de Puerto Rico
(2022-12-16)
En muchos países, una de las formas en las que las mujeres se han integrado a la política es ejerciendo funciones como primeras damas. Inés María Mendoza marcó en Puerto Rico el precedente de lo que se espera de dicha ...
An autoethnographic study on linguistic-communicative challenges and myths of court interpreting
(2023-05-11)
The role of the interpreter is to facilitate understanding between two or more persons who do not share the same language. In the legal system, that understanding requires accuracy, completeness, impartiality, and maintaining ...
Hurricane disturbance effects on tropical forests; structure, plant dynamics, and carbon storage
(2022-11-29)
Tropical cyclones are likely to increase in intensity, cause increased rainfall, and have larger storm surges. Changes in intensity and strength of tropical cyclones potentially have considerable effects on tropical forests. ...
Holothurian primary muscle cell culture: Optimization of cell culture techniques and elucidation of molecular pathways involved in muscle cell dedifferentiation
(2022-12-16)
Members of the phylum Echinodermata possess an impressive regenerative potential that could further develop the field of regenerative medicine. Holothurians have one of the most developed musculatures in terms of the myogenesis that leads to regeneration of muscle tissue. We aimed to develop an in vitro model for dissociated muscle tissue that could be used for regenerative studies. We focus on molecular candidates that have been previously associated with regeneration, to define their roles in the dedifferentiation of muscle cells. To do this, we optimized protocols for intestinal tissue cultures by changing parameters for ones that best suited our muscle tissue. Then, we applied pharmacological agents and analyzed the presence of SLS's and dedifferentiating fibers, structures that have been associated with the process of dedifferentiation, to elucidate which molecular pathways could induce the dedifferentiation of muscle cells. We found specific parameters such as changes in the enzymatic dissociation media, the adherence protocol and the number of cells cultured, that yielded healthy muscle fibers. Our cultures also showed a cellular component that was added to structures that indicated that dedifferentiation was occurring. For pharmacological assays, we found an increase in dedifferentiating fibers when we treated cultures with LiCl and an increase in SLS's when treated with DPI, EGTA and UK-383,367. Our results indicate that our in vitro model serves as an effective way to determine the effects that pharmacological agents have on dedifferentiation. We conclude that pathways involving Wnt/β- catenin and GSK-3, reactive oxygen species, Ca<sup>2+</sup> and BMP-1 modulate in vitro dedifferentiation of muscle fibers....
Del privilegio a los márgenes : la evolución de la representación de los personajes femeninos en Papeles de Pandora de Rosario Ferré, Cuentos traidores de Rubis Camacho y las Negras de Yolanda Arroyo Pizarro
(2023-12-19)
En la Década del Setenta del siglo XX, la literatura escrita por mujeres revolucionó la literatura puertorriqueña. Con Papeles de Pandora de Rosario Ferré da inicio la literatura feminista en Puerto Rico. En ese primer ...
El trabajo a distancia en Trabajo Social durante la pandemia del Covid-19: Condiciones laborales, acción profesional y conciliación familia-trabajo
(2023-12-04)
El presente estudio surge con la finalidad de recopilar las experiencias y aprendizajes de profesionales colegiados del trabajo social en teletrabajo y trabajo remoto durante la pandemia de COVID-19 y sus implicaciones ...
Iluminaciones en la sombra de Alejandro Sawa : modernismo y vanguardia en el "fin de siglo" español
(2023-12-06)
En esta investigación sobre el libro póstumo del emblemático bohemio español Alejandro Sawa, Iluminaciones en la sombra (1910), se revisa particularmente la categoría genérica de diario a la que tradicionalmente ha sido ...
El cambio de la educación presencial a la virtual en el contexto de la emergencia ocasionada por la pandemia del COVID 19: Estudio de caso de un centro preescolar en Puerto Rico
(2023-12-06)
Investigación de naturaleza cualitativa con un enfoque de estudio de caso. Esta investigación se centra en el cambio de la educación presencial a la virtual en un centro preescolar n Puerto Rico durante la emergencia del ...
Attention networks and attentional bias in young adults with mathematics anxiety
(2023-12-06)
Mathematics Anxiety (MA) is a debilitating negative reaction to arithmetic that affects up to 61% of students of all ages worldwide. It is defined as feelings of tension that affect the handling of numbers and the solving ...