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dc.contributor.authorPasten, Consuelo
dc.contributor.authorOrtiz-Pineda, Pablo A.
dc.contributor.authorGarcía-Arrarás, José E.
dc.date.accessioned2017-05-31T18:17:23Z
dc.date.available2017-05-31T18:17:23Z
dc.date.copyrightAll of the material available from the PMC site is provided by the respective publishers or authors (Dr. José E. García-Arrarás). Almost all of it is protected by U.S. and/or foreign copyright laws, even though PMC provides free access to it. The respective copyright holders retain rights for reproduction, redistribution and reuse. Users of PMC are directly and solely responsible for compliance with copyright restrictions and are expected to adhere to the terms and conditions defined by the copyright holder.en_US
dc.date.issued2012-04
dc.identifier.citationPasten, C., Ortiz-Pineda, P. A., & E.García-Arrarás, J. (2012). Ubiquitin-proteasome system components are up-regulated during intestinal regeneration. Genesis (New York, N.y. : 2000), 50(4), 350–365. http://doi.org/10.1002/dvg.20803en_US
dc.identifier.issn1526-968X
dc.identifier.urihttp://hdl.handle.net/11721/1621
dc.description.abstractThe ubiquitin proteasome system (UPS) is the main proteolytic system of cells. Recent evidence suggests that the UPS plays a regulatory role in regeneration processes. Here we explore the possibility that the UPS is involved during intestinal regeneration of the sea cucumber Holothuria glaberrima. These organisms can regenerate most of their digestive tract following a process of evisceration. Initially, we identified components of H. glaberrima UPS, including sequences for Rpn10, β3 and ubiquitin-RPL40. Predicted proteins from the mRNA sequences showed high degree of conservation that ranged from 60% (Rpn10) to 98% (Ub-RPL40). Microarrays and RT-PCR experiments showed that these genes were up-regulated during intestinal regeneration. In addition, we demonstrated expression of alpha 20S proteasome subunits and ubiquitinated proteins during intestinal regeneration and detected them in the epithelium and connective tissue of the regenerating intestine. Finally, the intestinal regeneration was altered in animals treated with MG132, a proteasome inhibitor. These findings support our contention that proteasomes are playing an important role during intestinal regeneration.en_US
dc.description.sponsorshipGrant sponsor: NIH-NIGMS Grant number: 1SC1GM084770 and S06GM08102 National Institute of General Medical Sciences : NIGMS SC1 GM084770-03 || GM.en_US
dc.Format.extent3.47 MBen_US
dc.language.isoen_USen_US
dc.publisherJohn Wiley & Sons, Inc.en_US
dc.subjectEchinodermsen_US
dc.subjectSea cucumberen_US
dc.subjectRegenerationen_US
dc.subjectProtein degradationen_US
dc.subjectProteasomeen_US
dc.titleUbiquitin-Proteasome System Components are Up-Regulated During Intestinal Regenerationen_US
dc.typeArticleen_US
dc.rights.licenceArticles that are available through the PMC OAI and FTP services are still protected by copyright but are distributed under a Creative Commons or similar license that generally allows more liberal use than a traditional copyrighted work. Please refer to the license statement in each article for specific terms of use. The license terms are not identical for all the articles.en_US
dcterms.licenseDr. José E. García-Arrarás, Department of Biology, PO Box 23360, University of Puerto Rico, San Juan, PR 00931. jegarcia@hpcf.upr.edu. Articles that are available through the PMC OAI and FTP services are still protected by copyright but are distributed under a Creative Commons or similar license that generally allows more liberal use than a traditional copyrighted work.en_US
dcterms.rightsDr. José E. García-Arrarás, Department of Biology, PO Box 23360, University of Puerto Rico, San Juan, PR 00931. jegarcia@hpcf.upr.edu.en_US
dcterms.rightsHolderDr. José E. García-Arrarás, Department of Biology, PO Box 23360, University of Puerto Rico, San Juan, PR 00931. jegarcia@hpcf.upr.edu.en_US
dc.identifier.doiDOI: 10.1002/dvg.20803en_US
dc.local.DepartmentDepartment of Biologyen_US
dc.local.FacultyCollege of Natural Sciencesen_US


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