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dc.contributor.authorQuesada, Orestes
dc.contributor.authorGonzález-Freire, Carol
dc.contributor.authorColón-Sáez, José O.
dc.contributor.authorFernández-García, Emily
dc.contributor.authorMercado, Juan
dc.contributor.authorDávila, Alejandro
dc.contributor.authorMorales, Reginald
dc.contributor.authorLasalde-Dominicci, José A.
dc.date.accessioned2017-03-23T22:25:51Z
dc.date.available2017-03-23T22:25:51Z
dc.date.copyrightOpen — authors articles are freely available to all and as the author, authors retain copyright.en_USA
dc.date.issued2016-09-19
dc.identifier.citationQuesada, Orestes et al. “Uncovering the Lipidic Basis for the Preparation of Functional Nicotinic Acetylcholine Receptor Detergent Complexes for Structural Studies.” Scientific Reports 6 (2016): 32766. doi:10.1038/srep32766.en_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://hdl.handle.net/11721/1571
dc.description.abstractThis study compares the lipid composition, including individual phospholipid molecular species of solubilized nAChR detergent complexes (nAChR-DCs) with those of the bulk lipids from their source, Torpedo californica (Tc) electric tissue. This lipidomic analysis revealed seventy-seven (77) phospholipid species in the Tc tissue. Analysis of affinity-purified nAChR-DCs prepared with C-12 to C-16 phospholipid analog detergents alkylphosphocholine (FC) and lysofoscholine (LFC) demonstrated that nAChRDCs prepared with FC12, LFC14, and LFC16 contained >60 phospholipids/nAChR, which was more than twice of those prepared with FC14, FC16, and LFC12. Significantly, all the nAChR-DCs lacked ethanolamine and anionic phospholipids, contained only four cholesterol molecules, and a limited number of phospholipid molecular species per nAChR. Upon incorporation into oocytes, FC12 produce significant functionality, whereas LFC14 and LFC16 nAChR-DCs displayed an increased functionality as compared to the crude Tc membrane. All three nAChR-DCs displayed different degrees of alterations in macroscopic activation and desensitization kinetics.en_US
dc.description.sponsorshipThis work was supported by the National Institutes of Health (NIH) Grants; 1R01GM098343 (JALD and OQ), Neuroimaging and Electrophysiology Facility Grant NIH P20 GM 103642 (JALD and OQ), Minority Access to Research Careers; (MARC) 5T34GM07821 (RM and OQ) and Research Initiative for Scientific Enhancement (RISE) 5R25GM061151 (RM and OQ) University of Puerto Rico Río Piedras Campus Institutional Funds for Research (JALD and OQ).en_USA
dc.Format.extent710.74 KBen_USA
dc.language.isoen_USen_US
dc.publisherNature / Scientific Reportsen_US
dc.titleUncovering the lipidic basis for the preparation of functional nicotinic acetylcholine receptor detergent complexes for structural studies.en_US
dc.typeArticleen_US
dc.rights.licenceCC BY license (Creative Commons Attribution 4.0 International License). All papers are available to the entire scientific community immediately after publication, with no barriers to access.en_USA
dcterms.licenseCC BY license (Creative Commons Attribution 4.0 International License). All papers are available to the entire scientific community immediately after publication, with no barriers to access.en_USA
dcterms.rightsOpen — authors articles are freely available to all and as the author, authors retain copyright.en_USA
dcterms.rightsHolderOpen — authors articles are freely available to all and as the author, authors retain copyright.en_USA
dc.description.doi10.1038/srep32766en_USA
dc.local.DepartmentDepartment of Biologyen_USA
dc.local.FacultyCollege of Natural Sciencesen_USA


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